The Influence of Cochlear Implant-Based Electric Stimulation on the Electrophysiological Characteristics of Cultured Spiral Ganglion Neurons

Figure 1

The cultured spiral ganglion neurons (SGNs) are treated with charge-balanced biphasic electrical stimulation. (a) Four-well chamber slides were used in this culture system. Four holes adjacent to the floor were made on two opposite walls of each chamber to introduce two platinum-iridium wires at the two opposite borders. The wires were connected to charge-balanced biphasic pulse generators. (b) The biphasic pluses used for the electrical stimulation were 50 μA or 100 μA amplitude, 65 μs pulse width, 8 μs open-circuit interphase gap, 4862 μs short-circuit phase, and 200 Hz frequency.

(a)

(b)