Cyclin-Dependent Kinase-Like 5 (CDKL5): Possible Cellular Signalling Targets and Involvement in CDKL5 Deficiency Disorder

Figure 2

Phosphorylation of Amph1 by CDKL5 and its biological importance at the synapse. (a) Amph1 binds to endophilin, an endocytosis-related protein. Phosphorylation of a Ser-293 residue in the RPRSP sequence of Amph1 results in the loss of this binding ability in vitro. (b) The predicted biological importance of Amph1 phosphorylation at the synapse. At the presynaptic membrane, synaptic vesicles release neurotransmitters into the synaptic cleft and are recycled by endocytosis. A complex centred around dynamin is believed to facilitate invagination and separation of the membrane during vesicle recycling. Because Amph1 and endophilin are included in this complex, CDKL5-mediated phosphorylation of Amph1 is predicted to either positively or negatively affect membrane recycling.

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