Research Article

Atrial Natriuretic Peptide Improves Neurite Outgrowth from Spiral Ganglion Neurons In Vitro through a cGMP-Dependent Manner

Figure 2

Immunolocalization of ANP, NPR-A, and NPR-C in dissociated SGNs from P3 rats. Merge and single-channel images of SG cells triple labeled with antibodies against TUJ1 (green), ANP/NPR-A/NPR-C (red), and DAPI (blue). The immunoreactivity of ANP (a), NPR-A (b), or NPR-C (c) was colocalized with β-III tubulin-positive SGNs, respectively, distributed in the neuronal soma and neurites. .