An Enriched Environment Enhances Angiogenesis Surrounding the Cingulum in Ischaemic Stroke Rats

Figure 8

Almost all CD34-positive cells surrounding the contralateral cingulate in MCAO/r rats were colabelled with eNOS, and almost all eNOS-positive cells surrounding the contralateral cingulate in MCAO/r rats were colabelled with VEGFR2. (a–f) Almost all CD34-positive cells surrounding the contralateral cingulate in MCAO/r rats were colabelled with eNOS. (a, d) Representative images of CD34-positive endothelial cells surrounding the contralateral cingulate in MCAO/r rats in the enriched environment (a) and standard cage groups (d). (b, e) Representative images of eNOS-positive endothelial cells surrounding the contralateral cingulate in MCAO/r rats in the enriched environment (b) and standard cage groups (e). (c, f) Almost all CD34-positive cells surrounding the contralateral cingulate in MCAO/r rats in the enriched environment (c) and standard cage groups (f) were colabelled with HIF-1α. (g–l) Almost all eNOS-positive cells surrounding the contralateral cingulate in MCAO/r rats were colabelled with VEGFR2. (g, j) Representative images of VEGFR2-positive endothelial cells surrounding the contralateral cingulate in MCAO/r rats in the enriched environment (g) and standard cage groups (j). (h, k) Representative images of eNOS-positive endothelial cells surrounding the contralateral cingulate in MCAO/r rats in the enriched environment (h) and standard cage groups (k). (i, l) Almost all eNOS-positive cells surrounding the contralateral cingulate in MCAO/r rats in the enriched environment (i) and standard cage groups (l) were colabelled with VEGFR2. .