Electroacupuncture Pretreatment Elicits Tolerance to Cerebral Ischemia/Reperfusion through Inhibition of the GluN2B/m-Calpain/p38 MAPK Proapoptotic Pathway

Figure 2

Effect of EA pretreatment on Garcia score, cerebral infarction volume, and the number of neuron apoptosis in the hippocampal CA1 region of MCAO/R rats. After cerebral I/R injury for 24 h, the neural behavior of the rats was evaluated by the Garcia score (), the infarct volume was detected by TTC staining of the brain slices (), and the cellular outcome was detected by TUNEL fluorescence staining (). (a) Comparison of Garcia score. The results conformed to the normal distribution yet with heterogenous variance. Differences among groups were examined using the Kruskal-Wallis test (, ) followed by the Dunn test for post hoc multiple comparisons. (b) Representative slice of TTC staining. (c) Comparison of infarct volume. The results conformed to the normal distribution with homogeneous variance. Differences among groups were examined using AVONA (, ) followed by LSD for post hoc multiple comparisons. (d) Representative immunofluorescence staining of TUNEL-positive cells (red) in brain sections. . (e) Comparison of apoptotic cell. The results conformed to the normal distribution and homogeneous variances. Differences among groups were examined using AVONA (, ) followed by LSD for post hoc multiple comparisons. vs. sham; ^# vs. MCAO/R.

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