Research Article

Electroacupuncture Pretreatment Elicits Tolerance to Cerebral Ischemia/Reperfusion through Inhibition of the GluN2B/m-Calpain/p38 MAPK Proapoptotic Pathway

Figure 4

Effect of EA pretreatment on m-calpain expressed in the rat hippocampal CA1 neurons (). The expression and cell localization of m-calpain at 24 h after cerebral I/R were detected by WB and double-standard immunofluorescence staining. (a) Representative WB bands showing m-calpain expression in rats among the three groups. (b) Comparison of the m-calpain expression. The result did not conform to the normal distribution. Differences among groups were examined using the Kruskal-Wallis test (.421, ) followed by the Dunn test for post hoc multiple comparisons. (c) Representative double immunofluorescence staining (yellow) of m-calpain-positive cells (red) and NeuN-positive cells (green) in brain sections. . vs. sham; # vs. MCAO/R.