Electroacupuncture Pretreatment Elicits Tolerance to Cerebral Ischemia/Reperfusion through Inhibition of the GluN2B/m-Calpain/p38 MAPK Proapoptotic Pathway

Figure 5

Effect of EA pretreatment on t-p38 and p-p38 expression in the hippocampal CA1 neurons (). The expression of p38 and p-p38 in the hippocampal neurons was detected by WB at 24 h after cerebral I/R. (a) Representative WB bands showing p38 MAPK expression in the rat hippocampus of three groups. (b, c) Comparison of total p38 (t-p38) and phophorylated p38 (p-p38) expression. The result of t-p38/β-tubulin conformed to normal distribution with homogeneous variance. Differences among groups were examined using AVONA (, ). The result of p-p38/β-tubulin conformed to normal distribution with homogeneous variance. Differences among groups were examined using AVONA (, ). (d) Comparison of p-p38/t-p38 ratio. The result of p-p38/t-p38 conformed to normal distribution with homogeneous variance. Differences among groups were examined using AVONA (, ) followed by LSD for post hoc multiple comparisons. vs. sham; ^# vs. MCAO/R.

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