GPER-Deficient Rats Exhibit Lower Serum Corticosterone Level and Increased Anxiety-Like Behavior

Figure 10

GPER-deficient rats exhibit anxiety-like behavior. (a) Comparison of the open-arm time and open-arm visits in the elevated plus maze (EPM) between WT and GPER^-/- rats of three age groups (10 weeks old, ; 16 weeks old, ; and 22 weeks old, ). (b) Comparison of the latency to drink and corner visits after punitive air puff in the IntelliCage between WT () and GPER^-/- () female rats. (c) Comparison of the center time, center distance, and center visits in the open field test between WT and GPER^-/- rats of three age groups (10 w, ; 16 w, ; and 22 w, ). (d) Protocol of the OVX test to further explore the role of GPER in modulation of anxiety. (e) Comparison of the open-arm time and open-arm visits in the EPM test among control (), ovariectomized (OVX, ), and OVX rats treated with E2 () or G-1 (). Note that OVX rats showed anxiety-like behaviors, which were reversed by E2 and G-1. (f) Comparison of the center time, center distance, and center visits in the open field test among control (), OVX (), and OVX rats treated with E2 () or G-1 (). Note that E2 and G-1 can reverse OVX-induced anxiety-like behaviors. , , and , two-way ANOVA with Bonferroni posttests for elevated plus maze and open field, unpaired -test for IntelliCage.

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