Effects of Mating and Social Exposure on Cell Proliferation in the Adult Male Prairie Vole (Microtus ochrogaster)

Figure 6

Photomicrographs of nuclei stained with Hoechst showing DG of the hippocampus in the male vole brain (a). Immunolabeling for BrdU in the DG from the control (C), social exposure to a female (SE m/f), social exposure to a male (SE m/m), and social cohabitation with mating (SCM) animals, respectively (b–e). White arrowheads point to single BrdU-positive cells, and white arrows indicate clusters of BrdU-positive cells. Scattered squares represent enlargements of the inserts at 20x, which show clustering of proliferating cells in the DG from the SE m/f and SCM voles. Data analysis for cell proliferation in the whole DG, including the hilus, for the C, SE m/f, SE m/m, and SCM animals (f). Data were analyzed with one-way ANOVA followed by Bonferroni’s post hoc tests. sp: suprapyramidal blade; ip: infrapyramidal blade; h: hilus. 20x. Scale bar: 100 μm. , , and . Results of ANOVA analyses are provided in the text. C (), SE m/f (), SE m/m (), and SCM ().

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