Annexin A3 as a Marker Protein for Microglia in the Central Nervous System of Rats

Figure 5

Morphologies of microglia labeled by ANXA3 and ANXA3 protein expression levels in different regions. (a) Low-magnification micrographs showing microglial cells in the ischemic penumbra 3 days after reperfusion. The yellow-dotted ring indicates the ischemic penumbra area between the infarct area and the normal area of the brain tissue. . (b) Representative immunofluorescence images showing the morphology of activated microglia in the ischemic penumbra (on the left) and resting microglia in the healthy area (on the right) labeled by ANXA3 and Iba1 at high magnification. . per group. (c, d) The expression levels of ANXA3, CD11b, and Iba1 proteins in the olfactory bulb, cortex, hippocampus, thalamus, cerebellum, and spinal cord of adult rats (3 months) were determined by Western blotting analysis. The panel shows protein bands corresponding to ANXA3, CD11b, Iba1, and tubulin. The histogram shows the results of the densitometric analysis. The data are expressed as the and were analyzed by one-way ANOVA with Tukey’s post hoc test. and compared with the cerebellum group. ^## and ^### compared with the cerebellum group. ^&, ^&&, and ^&&& compared with the cerebellum group. per group.

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