Electroacupuncture Ameliorates Neuroinflammation-Mediated Cognitive Deficits through Inhibition of NLRP3 in Presenilin1/2 Conditional Double Knockout Mice

Figure 4

EA treatment reverses the robust upregulated levels of NLRP3 inflammasome in the hippocampus of PS cDKO mice. (a)–(c) Total RNA was isolated and subjected to quantitate the mRNA levels of NLRP3, ASC, and caspase-1 in the hip by qRT-PCR ( mice for each group). The mRNA levels were standardized based on the respective level of β-actin. Values were expressed as relative changes to WT mice, which was set to 1. (d) Representative Western blot of NLRP3, ASC, and caspase-1 in the hippocampus. (e)–(g) Quantification of Western blot for NLRP3, ASC, and caspase-1 in the hippocampus ( mice for each group). The protein levels of NLRP3, ASC, and caspase-1 were normalized with the levels of β-actin. The values were expressed as relative changes to the respective WT mice, which was set to 1. Data are the , , .

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