Neuroprotective Effects of ZiBuPiYin Recipe on db/db Mice via PI3K-Akt Signaling Pathway by Activating Grb2

Figure 3

The effect of ZBPYR on the expression of Grb2, Gab2, Akt, p-Akt, and GSK3β in mouse hippocampus. (a) Compared with the control group, the expression of Grb2 in the hippocampus of the DM group was reduced. However, Grb2 levels increased after ZBPYR administration. The total level of Gab2 in the hippocampus did not change significantly between the three groups. The expression of p-Gab2 (Tyr452) in the hippocampus of DM/ZBPYR mice was moderately increased. (b) The levels of p85 and p-Akt (Ser473) were reduced in DM, while ZBPYR enhanced p85 and Akt hyperphosphorylation. The total Akt level was not different in the hippocampus between the three groups. (c) DM group hippocampus Ser9-phosphorylated GSK3β decreased, while p-GSK3β (Ser9) levels in the DM/ZBPYR group hippocampus increased. Values are from 3 mice in each group. and compared to control; , , and compared to DM.

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