Neuroprotective Effects of ZiBuPiYin Recipe on db/db Mice via PI3K-Akt Signaling Pathway by Activating Grb2

Figure 4

The effect of ZBPYR on the expression of Grb2, Gab2, Akt, p-Akt, and Gsk3β in mouse cerebral cortex. (a) The expression of Grb2 and p-Gab2 (Tyr452) in the cerebral cortex of DM mice was significantly reduced, and Grb2 and p-Gab2 (Tyr452) were increased to varying degrees after ZBPYR treatment. There were no statistically significant changes in the total levels of Gab2 and Akt in the three groups. (b) Compared with control mice, p-Akt (Ser473) expression in the cerebral cortex of DM mice was significantly reduced, while that of DM/ZBPYR mice was significantly increased. Compared with the DM group, p85 was significantly increased in the cerebral cortex of the DM/ZBPYR group. (c) The degree of Ser9 phosphorylation of GSK3β in the cerebral cortex of the DM group was reduced to varying degrees, and the level of p-GSK3β (Ser9) was increased in the cerebral cortex of the DM/ZBPYR group. Values are from 3 mice in each group. , , and compared to control; and compared to DM.

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