Impact of Hepatoma-Derived Growth Factor Blockade on Resiniferatoxin-Induced Neuropathy

Figure 1

Serum HDGF is expressed higher in sciatica patients and rHDGF antibody increases pain threshold in RTX-treated rats. (a) Comparison of serum HDGF protein expression between sciatica patients and normal controls after ELISA analysis. The values detected from individuals were drawn as dots. The expression levels were shown as scattered plots; the middle line demonstrated the mean value. compared with normal controls. (b) The PC-12 cells were incubated with different concentrations of rHDGF antibody (0, 10, 20, 50, and 100 nM) and 500 μM H₂O₂. Values are expressed as percentages of viable cells. , compared with control group (without rHDGF antibody and H₂O₂). ^## compared with the control group treated with 500 μM H₂O₂. Data are expressed as the (). (c) After the rats were divided into three groups, neuropathy was induced by intraperitoneally administration of a single dose of RTX (50 μg/kg). In the RTX+rHDGF antibody group, 100 μg/kg rHDGF antibody was administered and applied 24 hours before RTX injection. The control group received no rHDGF antibody pretreatment and no RTX injection. Calibrated forceps test was used to test the mechanical threshold for pain before and 1, 2, 3, and 7 days after RTX injection. , compared with control group. ⁺⁺ compared with RTX+rHDGF antibody group. Data are expressed as the ( per group).

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