Impact of Hepatoma-Derived Growth Factor Blockade on Resiniferatoxin-Induced Neuropathy

Figure 2

Effect of rHDGF antibody on astrocytes in the L3/L5 spinal cord dorsal horn (laminae I-II) of RTX-treated rats. After the rats were divided into three groups, neuropathy was induced by intraperitoneally administration of a single dose of RTX (50 μg/kg). In the RTX+rHDGF antibody group, 100 μg/kg rHDGF antibody was administered and applied 24 hours before RTX injection. The control group received no rHDGF antibody pretreatment and no RTX injection. At 3 or 7 days after RTX injection and/or pretreated with rHDGF antibody, L3-L5 spinal cord dorsal horn were harvested, and the proliferation of astrocytes (GFAP) was detected through immunofluorescence staining (200x magnification). (a) Representative immunofluorescence staining image shown are from 3 to 5 sections stained at least from 6 rats for each group. White rectangle in (a) indicates the astrocyte morphology for high magnifications. (b) Changes in intensity. , . Data are expressed as the ( per group).

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