Impact of Hepatoma-Derived Growth Factor Blockade on Resiniferatoxin-Induced Neuropathy

Figure 3

Effect of rHDGF antibody on inflammatory cytokines in RTX-treated rats. After the rats were divided into three groups, neuropathy was induced by intraperitoneally administration of a single dose of RTX (50 μg/kg). In the RTX+rHDGF antibody group, 100 μg/kg rHDGF antibody was administered and applied 24 hours before RTX injection. The control group received no rHDGF antibody pretreatment and no RTX injection. At 3 or 7 days after RTX injection and/or pretreated with rHDGF antibody, L3-L5 dorsal root ganglion and spinal cord dorsal horn were harvested. Inflammatory cytokines were examined through RT-PCR or western blot analysis. (a) Gene expression of TNF-α and (b) IL-1β in dorsal root ganglion of rats. Gene expressions are ratios relative to GAPDH. . (c) iNOS expression in the spinal cord dorsal horn of rats. Representative Western blot results was shown. Expression levels were normalized to β-actin. . Data are expressed as the ( per group).

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