Canonical Wnt Signaling Pathway on Polarity Formation of Utricle Hair Cells

Figure 1

The experimental procedure. (a) RNA-sequencing of the utricle of E13.5 and P1 mice. (b) To conditionally knockout β-catenin, the β-catenin^f/f female mice were mated with β-catenin^f/f male mice with Sox2^CreER+; the pregnant mice were treated with tamoxifen injection at E11.5 and E12.5, and the utricle of the embryonic mice was harvested at E18.5. (c) Utricles of E13.5 mice were cultured in vitro with DMSO (control), LiCl (activator), and IWP2 (inhibitor).

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