Canonical Wnt Signaling Pathway on Polarity Formation of Utricle Hair Cells

Figure 3

Wnt signaling pathway is active in the E13.5 utricle and changes during utricle development. (a) RT-PCR shows that Wnt5a, Wnt7a, Wnt11, β-catenin, and Frizzled were expressed in the utricle of E13.5 mice. (b) Differentially expressed Wnt pathway-related genes between the E13.5 and P1 utricle. The red dots represent the upregulated genes based on an adjusted value < 0.05 and ; the green dots represent the downregulated genes based on an adjusted value < 0.05 and ; the blue spots represent genes with no significant difference in expression. (c) PPI network of E13.5 and P1 utricle. Circles represent genes. Lines represent interactions between gene-encoded proteins, and the top 10 rankings are listed. (d) The relative levels of APC, Wif1, Camk2d, and Fzd6 were analysed by RT-qPCR. The data presented are based on at least three independent experiments. The data are presented as the . , , .

(a)

(b)

(c)

(d)