Deletion of Clusterin Protects Cochlear Hair Cells against Hair Cell Aging and Ototoxicity

Figure 1

Construction of Clu knockout mice. (a) Schematic drawing showing the strategy used for Clu gene disruption. The target sites of clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 small guide RNAs (sgRNAs) in the Clu gene are indicated in red, and the deleted region of the Clu gene in knockout mice is indicated by dashes. The positions of RT-PCR primers are indicated by arrows. (b) PCR products were separated on a 1.5% agarose gel, and the expected band sizes for WT and knockout (KO) alleles were 1,063 and 480 bps, respectively. (c) Sequencing results for homozygotes. The black arrows represent wild-type sequences, and the red arrows represent sequences following the deleted fragment.

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