Deletion of Clusterin Protects Cochlear Hair Cells against Hair Cell Aging and Ototoxicity

Figure 5

Assessment of age-related morphology of hair cells and supporting cells. (a) Cochlear basilar membrane immunostaining in P2m, P4m, P6m, and P9m Clu^−/− mice. (b) Cochlear basilar membrane immunostaining in P2m, P4m, P6m, and P9m WT mice. The number of remaining hair cells in Clu^−/− mice was significantly higher than that in WT mice at the same age. (c) Quantification of OHCs and IHCs in mice at P2m, P4m, P6m, and P9m across all tested frequencies in WT and Clu^−/− mice. Data are presented as the . , . . (d) Quantification of supporting cells at P4m, P6m, and P9m in WT and Clu^−/− mice. The red box shows a typical section after three-dimensional reconstruction. The percentages of supporting cells and hair cells showed no significant differences between WT and Clu^−/− mice. .

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