[Retracted] Molecular Basis of GABA Hypofunction in Adolescent Schizophrenia-Like Animals

Figure 2

Electrophysiological recording of pyramidal neurons and PVIs⁺ in the mPFC in adolescence. (a) Schematic diagram of whole-cell patch-clamp of sIPSC obtained from pyramidal neurons in the PrL region of mPFC. Saline or MK-801-treated mice were sacrificed on ~PND 30 and subjected for electrophysiological recording. (b) Example traces of sIPSC in pyramidal neurons. (c) Cumulative probability and the average frequency of sIPSC (saline/MK801: cells from 3/3 mice per group, ). (d) Cumulative probability and average amplitude of sIPSC (saline/MK-801: cells from 3/3 mice per group, ). (e) Example traces of PVIs⁺ response to depolarizing current steps. (f) Input-output curve showing the average firing rate of PVIs⁺ in response to a series of increasing current steps. PVIs⁺ in the MK-801 group fired at an overall higher rate (two-way repeated-measures ANOVA, saline/MK-801: cells from 4/3 mice per group, ). (g) Average action potential threshold of PVIs⁺ (Student’s -test: saline/MK-801: cells from 4/3 mice per group, ). (h) Density of cells with PVIs⁺ immunoreactivity in the PrL region. Student’s -test: for each group, . (i) Western blot analysis of PV immunoreactivity: for the saline/MK-801 group. Student’s -test, . (j) Example traces of pyramidal neurons in response to depolarizing current steps. (k) Average action potential threshold of pyramidal neurons (saline/MK-801: cells from 3/4 mice per group, ). (l) Input-output curve showing the average firing rate of pyramidal neurons in response to a series of increasing current steps (two-way repeated-measures ANOVA, saline/MK-801: cells from 3/4 mice per group, ). (m) Example traces of sEPSC in pyramidal neurons. (n) Cumulative probability and the average amplitude of sEPSC (saline/MK801: cells from 4/3 mice per group, ). (o) Cumulative probability and the average frequency of sEPSC (saline/MK-801: cells from 4/3 mice per group, ). Data were represented as , . Student’s -test for two groups’ comparison.

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