[Retracted] Molecular Basis of GABA Hypofunction in Adolescent Schizophrenia-Like Animals

Figure 4

Integrative analysis of proteomics and single-cell sequencing data of differentially expressed proteins in adolescent mice. (a) All of the differentially expressed proteins in PFC identified in proteomics analysis were mapped to the mouse brain single-cell sequencing database (http://mousebrain.org). We downloaded the expression value of each differentially expressed protein within its clustered cell types in the cortex. A total of 45 cellular subtypes were showed, consisted of five major cell types of excitatory neurons, inhibitory neurons, astrocytes, oligodendrocytes, and microglia. Hierarchical clustering of the expression of significant proteins in all cell types showed their prominent cellular location. Red: higher protein expression. Blue: lower protein expression. Protein’s expression was scaled in a row and represented as score. Pie graphs on the right panel showed the number and percentage of downregulated proteins in each cell type. (b, c) Top GO enrichment of biological function of differentially expressed proteins in interneurons and astrocytes. Circle size represents the protein numbers in each item. (d) Heatmap of differentially expressed proteins involved in the metabolic processes in astrocytes and interneurons. Proteins associated with GABA signaling were from astrocytes and interneurons and downregulated after postnatal MK-801 injection. Each square represents one sample. Red: upregulated. Blue: downregulated. Protein’s expression was scaled in a row and represented as score.

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