ROS-Induced Oxidative Damage and Mitochondrial Dysfunction Mediated by Inhibition of SIRT3 in Cultured Cochlear Cells

Figure 1

H₂O₂ induces dose-dependent loss of ribbon synapses and hair cells at the 0, 0.25, 0.5 0.75, 1, and 1.25 mM concentrations. (a) Confocal images showing ribbon synapses (red) and hair cells (green) in the middle region of cochlear explants at each concentration. The white arrows indicate OHC damaged areas. Yellow arrows indicate IHC damaged areas. (b) Quantification of ribbon synapses at each concentration. (c) Rate of OHC survival at each concentration. (d) Rate of IHC survival at each concentration. Data are expressed as ( organ of Corti per concentration). , , and .  μm.

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