Astragaloside IV Alleviates Brain Injury Induced by Hypoxia via the Calpain-1 Signaling Pathway

Figure 3

AS-IV and calpain-1 knockout can reduce the oxidative stress of hippocampal CA1 neurons in mice with hypoxic brain injury. (a–c) The changes in SOD, MDA, and GSH-Px were measured by SOD, MDA, and GSH-Px determination kits, respectively (; SOD, ; MDA, ; GSH-Px, ). (d–e) The DHE probe labeling method was used to observe the production of reactive oxygen species in the hippocampal CA1 region of mice in each group (; ). (f) Morphological changes of hippocampal CA1 neurons observed by HE staining (). Data are presented as the . compared with the HBD group, ^## compared with the ASIV 60 mg/kg group.