Astragaloside IV Alleviates Brain Injury Induced by Hypoxia via the Calpain-1 Signaling Pathway

Figure 8

AS-IV inhibits hypoxia-induced activation of the calpain-1/HIF-1α/caspase-3 pathway in HT22 cells. (a, b) Calpain-1 expression in HT22 cells was observed by immunofluorescence (; ). (c–e) Western blotting was used to detect calpain-1 and HIF-1α protein expression (; calpain-1, ; HIF-1α, ). (f–h) After YC-1 was introduced, the protein expression of calpain-1 and caspase-3 was detected by Western blotting (; calpain-1, ; caspase-3, ). (i, j) After introducing YC-1, the expression of calpain-1 in HT22 cells was observed by immunofluorescence (; ). Data are presented as the . compared with the HMG group, ^#, ^## compared with the ASIV 50 μM group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)