Deficient Autophagy in Microglia Aggravates Repeated Social Defeat Stress-Induced Social Avoidance

Figure 4

Repeated social defeat stress enhanced autophagy activation in the prefrontal cortex of resilient mice. (a–e) Representative images (a) and quantitative analyses (b–e) of immunostaining for LC3B-puncta colocalized with p62 signals in the PFC of CX3CR1^GFP/+ mice without RSD (Con) and susceptible and resilient mice 24 h after the last session of RSD. In the merged images in (a), CX3CR1, LC3B, and p62 are shown in green, white, and red, respectively. Scale bars, 10 μm. (b) was determined by calculating the density of the LC3B area/total area. (c) was determined by calculating the density of the positive LC3B signals in each microglia. (e) was determined by calculating the density of the p62 area/total area. (f) was determined by calculating the density of the positive p62 signals in each microglia. (f–h) Representative images (f) and quantitative analyses (g, h) of immunostaining for FKBP5 in the PFC of Cx3CR1^GFP/+ mice without RSD (Con) and susceptible and resilient mice 24 h after the last session of RSD. In the merged images in (f), CX3CR1 and FKBP5 are shown in green and red, respectively. Scale bars, 10 μm. (g) was determined by calculating the density of the FKBP5 area/total area. (h) was determined by calculating the density of the positive FKBP5 signals in microglia. Con: control mice; Sus: susceptible mice; Re: resilient mice. One-way ANOVA followed by Turkey’s post hoc test was applied to all comparisons. Data are presented as the . Con, ; Sus, ; Res, . , , and vs. Con or Re.

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