Deficiency of MicroRNA-23-27-24 Clusters Exhibits the Impairment of Myelination in the Central Nervous System

Figure 3

Behavioral assessment. Wild-type (WT) and miR-23-27-24 cluster knockout (KO), per group. (a) Open field test. Total distance travelled by the mouse in 3 min was measured. In both WT and KO mice, the total distance travelled by the 10-week-old and 12-month-old mice was significantly more than the distance travelled by the 4-week-old mice. There was no significant difference between WT mice and KO mice at all ages. (b) Hanging wire test. The time until the mouse fell off from the wire was measured (duration to fall). In both WT and KO mice, the duration to fall was significantly shorter at 12 months of age than that at 4 or 10 weeks of age. In contrast, the duration to fall at 10 weeks of age was significantly longer than that at 4 weeks of age only in WT mice. The duration to fall in KO mice was significantly shorter than that in WT mice at 10 weeks of age. Compared to 4-week-old. Compared to 10-week-old. ^†Comparison between WT and KO mice, . (c) Balance beam test. The time taken for the mouse to cross the beam was measured (duration to reach). The duration to reach was significantly longer at 12 months of age than that at 4 or 10 weeks of age. There was no significant difference between WT mice and KO mice at all ages. Compared to 4-week-old. Compared to 10-week-old, .

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