Deficiency of MicroRNA-23-27-24 Clusters Exhibits the Impairment of Myelination in the Central Nervous System

Figure 8

Immunostaining of oligodendrocytes and their progenitor cells in brain sections. Wild-type (WT) and miR-23-27-24 cluster knockout (KO), per group. (a) Brain sections were immunostained for platelet-derived growth factor receptor α (PDGFRα), a marker of oligodendrocyte progenitors, CC1, a marker of mature oligodendrocytes, and OLIG2, a broad marker of oligodendrocytes. Scale bars: 50 μm. (b) Number of mature oligodendrocytes showing double positivity for CC1 and OLIG2. The number of mature oligodendrocytes at 12 months of age was significantly lower compared to that at 4 and 10 weeks of age in both WT and KO mice. The number of mature oligodendrocytes in KO mice was significantly lower than that in WT mice only at 12 months of age. Compared to 4-week-old. Compared to 10-week-old. ^†Comparison between WT and KO mice, . (c) Number of oligodendrocyte progenitors showing double positivity for PDGFRα and OLIG2. The number of oligodendrocyte progenitors at 12 months of age was significantly lower compared to that at 4 and 10 weeks of age in both WT and KO mice. However, there was no significant difference in the number of oligodendrocyte progenitors between WT and KO mice at all ages. Compared to 4-week-old. Compared to 10-week-old, .

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