Deficiency of MicroRNA-23-27-24 Clusters Exhibits the Impairment of Myelination in the Central Nervous System

Figure 9

Myelinating oligodendrocytes of the spinal cord and brain at 4 weeks of age. Wild-type (WT) and miR-23-27-24 cluster knockout (KO). (a) Sections of the spinal cord and brain were immunostained for myelin basic protein (MBP) and OLIG2. Cells that were double positive for MBP and OLIG2 were considered myelinating oligodendrocytes. Scale bars: 50 μm. (b) In the spinal cord, the ratio of the number of myelinating oligodendrocytes to the number of OLIG2-positive mature oligodendrocytes was significantly lower in KO mice than that in WT mice, per genotype, . (c) In the brain, the ratio of the number of myelinating oligodendrocytes to the number of OLIG2-positive mature oligodendrocytes was significantly lower in KO mice than that in WT mice, per genotype, . (d) Representative western blots of MBP and GAPDH proteins in the spinal cord of 4-week-old mice. MBP expression was significantly lower in KO mice than that in WT mice, per genotype, .

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