Administration of Recombinant Heat Shock Protein 70 Delays Peripheral Muscle Denervation in the SOD1G93A Mouse Model of Amyotrophic Lateral Sclerosis
Figure 2
rhHsp70 injections preserve larger axons in the L4VR. (a) There was no difference in the overall number of myelinated axons in the 4th lumbar ventral root at P30 or P75 although there appears to be a trend toward decreased numbers in the SOD1 groups at P75. Data are represented as percent of WT SEM at each time point. for each group. Statistical significance was determined by running an ANOVA, followed by the Tukey-Kramer multiple comparisons post-hoc test. (b) Although there was not a significant difference in the number of axons in treated and untreated mice, photomicrographs suggest that disease morphology of myelinated axons has progressed more in untreated mice as compared to treated animals. Photomicrographs of myelinated axons were taken at 40x. Scale bars = 40 μm. (c) Enlargements of photomicrographs of myelinated axons taken at 40x. Scale bars = 40 μm. (d) Size profiles of the axon area revealed a greater number of larger axons in WT animals ). Data are representative histograms of the average area of myelinated axons in the L4VR of SOD1-untreated and WT mice at P30. Statistical significance was determined by running a two-way ANOVA. Axons were traced using a camera lucida at 40x scanned and average axon area was determined using Scion Image software. Data are represented as the average number of axons per area range. for each group. (e) There were a greater number of larger axons in SOD1-treated and WT animals as compared to SOD1-untreated mice () at P75. There was no significant difference in the number of larger axons between SOD1-treated and WT animals. Data are representative histograms of the average number of axons per area range. for each group at P75. Statistical significance was determined using repeated measures (mixed model) ANOVA. Statistical significance was determined using repeated measures (mixed model) ANOVA.
