Short and Long-Term Analysis and Comparison of Neurodegeneration and Inflammatory Cell Response in the Ipsilateral and Contralateral Hemisphere of the Neonatal Mouse Brain after Hypoxia/Ischemia

Figure 10

Iba-1 immunostaining in control animals and H/I effects on the caudate-putamen (CP) (a–f) and neocortex (CX) (g–l). Control brain P7 shows basal expression of microglial Iba-1 in the CP (a) and CX (g). In the CP, activated microglia increase in the contralateral (CL) hemisphere at 3 hrs (b) up to 48 hrs (c). Higher activation is observed in the ipsilateral (IL) hemisphere at 3 hrs (d), showing a maximum at 48 hrs (e), and slowly returns to resting morphology with some patches of activated microglia after 14 days (f). Similar pattern is observed in the neocortex, CL hemisphere shows differential expression with respect to control animals from 3 hrs (h) to 72 hrs (i). Higher activation is observed in the ipsilateral (IL) hemisphere at 3 hrs (j), a clear amoeboid patch pattern is observed in the neocortex after 72 hrs (k), which slowly returns to resting morphology with some patches of primitive ramified microglia after 7 days (l). Scale bars: 50 μm.