Differential Expression of Tyrosine Hydroxylase Protein and Apoptosis-Related Genes in Differentiated and Undifferentiated SH-SY5Y Neuroblastoma Cells Treated with MPP⁺

Figure 6

Effect of MPP⁺ on p53 and caspase-3 mRNA expression in undifferentiated and differentiated SH-SY5Y cells. Cells were differentiated in 10-μM retinoic acid (RA) for 3 days. Undifferentiated (a and c) and differentiated cells (b and d) were exposed to 500 and 1000 μM of MPP⁺ for 24 hours, respectively. Expression of p53 mRNA (a and b) and caspase-3 (c and d) was analyzed with quantitative real-time RT-PCR, relatively compared to their respective untreated controls. The expression levels of the target gene were normalized to the expression level of β-actin. Data are expressed as mean ± SEM (). ; .

(a)

(b)

(c)

(d)