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Neurology Research International
Volume 2017, Article ID 5952149, 6 pages
https://doi.org/10.1155/2017/5952149
Research Article

Accumulation of α-Synuclein in Cerebellar Purkinje Cells of Diabetic Rats and Its Potential Relationship with Inflammation and Oxidative Stress Markers

1Department of Neurology, Trakya University Faculty of Medicine, Edirne, Turkey
2Department of Physiology, Kafkas University Faculty of Medicine, Kars, Turkey
3Department of Histology and Embryology, Ege University Faculty of Medicine, Izmir, Turkey
4Department of Physiology, Bilim University Faculty of Medicine, Istanbul, Turkey
5Department of Physiology, Ege University Faculty of Medicine, Izmir, Turkey

Correspondence should be addressed to Dilek Taşkıran; rt.ude.ege@nariksat.kelid

Received 29 August 2016; Accepted 20 December 2016; Published 4 January 2017

Academic Editor: Mamede de Carvalho

Copyright © 2017 Volkan Solmaz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. The present study was conducted to evaluate the relationship between plasma oxidative stress markers such as malondialdehyde (MDA) and glutathione (GSH), inflammatory marker pentraxin-3 (PTX3), and cerebellar accumulation of α-synuclein in streptozotocin- (STZ-) induced diabetes model in rats. Methods. Twelve rats were included in the study. Diabetes () was induced with a single intraperitoneal injection of streptozotocin (STZ, 60 mg/kg). Diabetes was verified after 48 h by measuring blood glucose levels. Six rats served as controls. Following 8 weeks, rats were sacrificed for biochemical and immunohistochemical evaluation. Results. Plasma MDA levels were significantly higher in diabetic rats when compared with the control rats (), while plasma GSH levels were lower in the diabetic group than in the control group (). Also, plasma pentraxin-3 levels were statistically higher in diabetic rats than in the control rats (). The analysis of cerebellar α-synuclein immunohistochemistry showed a significant increase in α-synuclein immunoexpression in the diabetic group compared to the control group (). Conclusion. Due to increased inflammation and oxidative stress in the chronic period of hyperglycemia linked to diabetes, there may be α-synuclein accumulation in the cerebellum and the plasma PTX3 levels may be assessed as an important biomarker of this situation.