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Obstetrics and Gynecology International
Volume 2010 (2010), Article ID 192461, 7 pages
http://dx.doi.org/10.1155/2010/192461
Research Article

CD9 Expression by Human Granulosa Cells and Platelets as a Predictor of Fertilization Success during IVF

1Department of Biology, Rhodes College, 2000 North Parkway, Memphis, TN 38112, USA
2Department of Obstetrics and Gynecology, University of Tennessee Health Science Center, Memphis, TN 38152, USA
3Department of Internal Medicine, The Vascular Biology Center of Excellence, University of Tennessee Health Science Center, Memphis, TN 38152, USA
4Fertility Associates of Memphis, 80 Humphreys Center, Suite 307, Memphis, TN 38120, USA

Received 27 May 2010; Revised 16 July 2010; Accepted 28 July 2010

Academic Editor: Edward V. Younglai

Copyright © 2010 Carolyn R. Jaslow et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To determine whether CD9 expression on human granulosa cells (GCs) and platelets could predict the success of conventional fertilization of human oocytes during in vitro fertilization (IVF). Methods. Thirty women undergoing IVF for nonmale factor infertility participated. Platelets from venous blood and GCs separated from retrieved oocytes were prepared for immunofluorescence. Flow cytometry quantified the percent of GCs expressing CD9, and CD9 surface density on GCs and platelets. Fertilization rate was determined for the total number of oocytes, and the number of mature oocytes per patient. Correlations tested for significant relationships ( ) between fertilization rates and CD9 expression. Results. CD9 surface density on human GCs is inversely correlated with fertilization rate of oocytes ( ), but the relationship was weak. Conclusion. More studies are needed to determine if CD9 expression on GCs would be useful for predicting conventional fertilization success during IVF.