Protein Kinase Cα Modulates Estrogen-Receptor-Dependent Transcription and Proliferation in Endometrial Cancer Cells
Figure 2
PKCα enhances ER-dependent promoter activity. Ishikawa cells were transiently transfected with (a) 0.5 μg pEREluc or (b) 0.5 μg pPS2luc and 0.3 μg pCMVβ in the presence or absence of 0.5 μg pmyrPKCα or vector control (pCDNA3). Cells were treated with ±100 nM estradiol (E2), as indicated. Luciferase activity was normalized to β-galactosidase and promoter activity expressed as fold increase over control. Data are mean ± s.e.m of 6 experiments conducted in triplicate.
