The Oncoplacental Gene Placenta-Specific Protein 1 Is Highly Expressed in Endometrial Tumors and Cell Lines

Table 2

Primer sequences used for conventional and quantitative PCR assays.

Amplicon	Size	Sequence	𝑇𝑚
Exon 6 coding region	812 bp	Forward: 5′-TCCTGTTTCCTGTGGTTCATT-3′	62.0°C
		Reverse: 5′-TCATGAAGTTGCTATAGGTTTCTCT-3′	62.0°C
Exon 5-6*	232 bp	Forward: 5′-CACCAGTGAGCACAAAGCCACATT-3′	60.3°C
		Reverse: 5′-CCATGAACCAGTCTATGGAG-3′	52.3°C
Exon 4-5-6^#	361 bp	Forward: 5′-GTGACTCTCCTATGAAGGTAAAGG-3′	54.4°C
		Reverse: 5′-CCATGAACCAGTCTATGGAG-3′	52.3°C
Exons 1-5-6§	283 bp	Forward: 5′-AAACTTACACGAGGAGTCTGTC-3′	57.2°C
		Reverse: 5′-CTGTGACCATGAACCAGTCTAT-3′	54.2°C
18S rRNA	104 bp	Forward: 5′-AACTTTCGATGGTAGTCGCCG-3′	57.3°C
		Reverse: 5′-CCTTGGATGTGGTAGCCGTTT-3′	57.6°C

∗qPCR assay for total PLAC1 mRNA.
^#qPCR assay for Promoter 2 transcribed PLAC1 mRNA.
§qPCR assay for Promoter 1 transcribed PLAC1 mRNA.