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Figure 6: Effects of 4-methylpyrazole (4-MP) treatment on ethanol-induced neurotoxicity. Control or ethanol exposed (50 mM) rat cerebellar neuron cultures seeded into 96-well plates were treated with vehicle or 4-MP for 48 h. The cells were used to measure (a) 4-HNE, (b) 8-OHdG, (c) Cyquant fluorescence-viability (CYQ), (d) ATP content, (e) MitoTracker Red (MTR), (f) MitoTracker Green (MTG), (g) ChAT, and (h) GAPDH. 4-HNE, 8-OHdG, ChAT, and GAPDH immunoreactivities were measured by cellular ELISA. Viability and mitochondrial assays were measured in labeled cells. All results were normalized to H33342 fluorescence. Graphs depict the mean ± S.D. of results. Statistical comparisons were made using two-way ANOVA with the post hoc Bonferroni significance test. Significant differences are shown within each panel. ** and *** between control and ethanol-exposed cells within the vehicle- or 4-MP treated groups.