4-Hydroxy-2-Nonenal-Modified Glyceraldehyde-3-Phosphate Dehydrogenase Is Degraded by Cathepsin G in Rat Neutrophils

Figure 1

Concentration-dependent effect of HNE on degradation of GAPDH by incubation with cell extracts from neutrophils. The cell extract from neutrophils was incubated with eGAPDH at the indicated concentrations of HNE at 37°C for 3 h. Reaction products were separated on 12% SDS-PAGE gels and analyzed by Western blotting using an anti-GAPDH mAb. Data are mean ± SE (bars) values from four independent experiments. Significant decreases in GAPDH level compared to control are indicated by asterisks: *𝑃<0.05, **𝑃<0.01, and ***𝑃<0.001.