S-Nitrosation of Cellular Proteins by NO Donors in Rat Embryonic Fibroblast 3Y1 Cells: Factors Affecting S-Nitrosation

Figure 4

Effects of FCS, BSA, and NEM-BSA on CysNO-mediated S-nitrosation in 3Y1 cells. Cells maintained in HBSS or DMEM in the absence or presence of FCS (10%), BSA (3 mg/ml), or NEM-treated BSA (3 mg/ml) were treated with CysNO (200 μM) for 30 min. The levels of S-nitrosated proteins were measured by the DAN assay, and the value for cells treated with CysNO in HBSS alone (none) was taken as 100% (12 ± 2 nmol/mg protein). Values are mean ± SEM of three to four independent assays. *𝑃<0.001 versus none; ^#𝑃<0.001 versus BSA.