Activation of Nrf2-Regulated Glutathione Pathway Genes by Ischemic Preconditioning
Upregulation of glutathione pathway components following in vivo ischemic preconditioning. Gene expression of glutathione pathway components, Gclc (a), xCT (b), and Gclm (c), was quantified by qPCR in mice subjected to a transient 15-minute occlusion of the middle cerebral artery, a stimulus known to trigger neuroprotective preconditioning in vivo. Transient ischemia significantly increased the cortical expression of Gclc, xCT, and Gclm in the ipsilateral hemisphere, as compared to the contralateral hemisphere, suggesting a specific recruitment of the ARE/Nrf2/glutathione pathway in the protective effects of preconditioning in vivo (bars represent mean ± SEM,**, ***, Student’s -test, ).
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