Effect of Novel Marine Nutraceuticals on IL-1α-Mediated TNF-α Release from UVB-Irradiated Human Melanocyte-Derived Cells
Figure 4
Effect of test compounds on the expression of phospho-p38 protein in UVB-irradiated (2 kJ/m2) (a and b) HEM and (c and d) MM96L cells. Cell cultures were incubated with 0.625 μg/mL of test compounds for 24 h prior to and 15 min after UVB-irradiation with or without 10 ng/mL IL-1α. The proteins were extracted at 15 min after-irradiation and western blotting was performed. A representative western blot probed for phospho-p38 MAPK in (b) HEM and (d) MM96L cells incubated with either α-tocopherol, CO2-SFE mussel oil, or 5β-scymnol prior to and after UVB-irradiation. The sham-irradiated control (lanes 1–8) or cell cultures exposed to high-dose UVB radiation (lanes 9–16) were treated with 10 ng/mL IL-1α (lanes 2, 4, 6, 8, 10, 12, 14, and 16). Lanes 1, 2, 9, 10: no test compound, lanes 3, 4, 11, 12: 0.625 μg/mL α-tocopherol, lanes 5, 6, 13, 14 : 0.625 μg/mL CO2-SFE mussel oil, and lanes 7, 8, 15, 16: 0.625 μg/mL 5β-scymnol. Results expressed as the means ± SD of triplicate samples. Statistical analysis was performed using Student’s paired t-test where significance was recorded as . (*) Significant difference between untreated sample and test compound-treated sample.
