Oxidative Medicine and Cellular Longevity / 2011 / Article / Fig 4

Research Article

Role of Reactive Oxygen Species in the Neural and Hormonal Regulation of the PNMT Gene in PC12 Cells

Figure 4

Effect of ROS on the neural regulation of the PNMT gene. PC12 cells were treated with 200 μM CoCl2, 10 μM forskolin or the combination of CoCl2 and forskolin for 24 h at 37°C. (a) Luciferase activity from cells transfected with the wild-type pGL3RP893 PNMT promoter luciferase reporter gene construct. (b) Representative image ( ) of PNMT (intron-retaining and intronless splice variants) mRNA levels from semi-quantitative RT-PCR. GAPDH mRNA was used as a housekeeping control. (c) PNMT intron-retaining mRNA and (d) intronless mRNA. Data is normalized to GAPDH control. Data presented as relative fold change ± SEM from a minimum of three independent experiments. Significance defined as * ; **/++ ; +++/### .
(a) Transfection
(b) Representative gel image
(c) PNMT intron-retaining mRNA
(d) PNMT intronless mRNA

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