Role of Reactive Oxygen Species in the Neural and Hormonal Regulation of the PNMT Gene in PC12 Cells
Table 1
The following primer sets were used for PNMT and glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
Gene
Species
Accession number
Primer sequence (5′–3′)
Amplicon
No. of cycles
Annealing temp. (°C)
PNMT
R. norvegicus
X75333
CAGACTTCTTGGAGGTCAACCG
422 bp, 312 bp
35
58
(forward)
AGCAGCGTCGTGATATGATAC
(reverse)
GAPDH
R. norvegicus
BC059110
ATGGTGGTGCTGAGTATGTCG
475 bp
21
58
(forward)
CATGTCAGATCCACAACGGATAC
(reverse)
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