Role of Oxidative Stress in Transformation Induced by Metal Mixture

Figure 3

Effects of the metal mixture through initiation phase of transformation process, antioxidant activity markers. Balb/c 3T3 cells were exposed to initiator stimuli, metal mixture (2 𝜇M NaAsO₂, 2 𝜇M CdCl₂, and 5 𝜇M Pb(C₂H₃O₂)₂·3H₂O) or MNNG (positive initiator), on day 1. Samplings were on days 1, after 4 hours of initiator exposure, and on days 4 and 7 before changing medium. Data represent the mean of 3 individual experiments performed by triplicate. Antioxidant activity is represented as a percentage with respect to control values. Superoxide dismutase (SOD) and catalase activities were evaluated using spectrophotometric assays, and the total antioxidant capacity (TAC) was assessed by the ABTS° + radical method (ANOVA and Student’s t-test; *𝑃<0.05, **𝑃<0.001, ***𝑃<0.0001 versus control bars).