Role of Oxidative Stress in Transformation Induced by Metal
Mixture
Table 2
Transforming potential through promotion phase.
Promotion Phase
Day
Treatmenta
Viability (%)b
Dish with foci/dish scoredc
No. of transformed foci/dish
TPd
11
Control
100 ± 0.5
4/6
1.8 ± 0.05
1 ± 0.27
MNNG + TPA
77 ± 19.1*
6/6
62.25 ± 9.9***
61.6 ± 9.8***
Metal mixture + TPA
57 ± 15.8*
6/6
33.75 ± 11.6***
62 ± 21.3***
MNNG + Meta mixture
9 ± 0.66***
6/6
23 ± 7.7***
70.5 ± 23.8***
Metal mixture + metal mixture
17 ± 0.3***
6/6
29.5 ± 5.7***
78.5 ± 15.1***
16
Control
100 ± 0.0
4/6
1.8 ± 0.05
1 ± 0.27
MNNG + TPA
100 ± 11.5
6/6
62.25 ± 9.9***
67.9 ± 10.8***
Metal mixture + TPA
100 ± 18.5
6/6
33.75 ± 11.6***
25.1 ± 8.6***
MNNG + metal mixture
31 ± 8.9**
6/6
23 ± 7.7***
38.5 ± 13***
Metal mixture + metal mixture
30 ± 8***
6/6
29.5 ± 5.7***
55.2 ± 10.6***
21
Control
100 ± 0.0
4/6
1.8 ± 0.05
1 ± 0.27
MNNG + TPA
77 ± 0.5***
6/6
62.25 ± 9.9***
42.6 ± 6.8***
Metal mixture + TPA
88 ± 2.2**
6/6
33.75 ± 11.6***
11.7 ± 4*
MNNG + metal mixture
76 ± 0.05***
6/6
23 ± 7.7***
13.6 ± 4.6*
Metal mixture + metal mixture
31 ± 6.9***
6/6
29.5 ± 5.7***
50.5 ± 9.7***
aTreatments were added at concentrations of MNNG 0.5 g/mL, TPA 0.1 g/mL, and metal mixture (As 2 M, Cd 2 M, and Pb 5 M); bpercentage with respect to controls; ctwo experiments with three biological replicates; dtransforming potential (TP) calculated as nmber of transformed foci type III per dish/surviving cells at corresponding sampling day. Statistical analysis, Student’s t-test value *< 0.05, **< 0.01 and ***< 0.001.
