Table 2: Transforming potential through promotion phase.

Promotion Phase
DayTreatmentaViability (%)bDish with foci/dish scoredcNo. of transformed foci/dishTPd

11Control100 ± 0.54/61.8 ± 0.051 ± 0.27
MNNG + TPA77 ± 19.1*6/662.25 ± 9.9***61.6 ± 9.8***
Metal mixture + TPA57 ± 15.8*6/633.75 ± 11.6***62 ± 21.3***
MNNG + Meta mixture9 ± 0.66***6/623 ± 7.7***70.5 ± 23.8***
Metal mixture + metal mixture17 ± 0.3***6/629.5 ± 5.7***78.5 ± 15.1***
16Control100 ± 0.04/61.8 ± 0.051 ± 0.27
MNNG + TPA100 ± 11.56/662.25 ± 9.9***67.9 ± 10.8***
Metal mixture + TPA100 ± 18.56/633.75 ± 11.6***25.1 ± 8.6***
MNNG + metal mixture31 ± 8.9**6/623 ± 7.7***38.5 ± 13***
Metal mixture + metal mixture30 ± 8***6/629.5 ± 5.7***55.2 ± 10.6***
21Control100 ± 0.04/61.8 ± 0.051 ± 0.27
MNNG + TPA77 ± 0.5***6/662.25 ± 9.9***42.6 ± 6.8***
Metal mixture + TPA88 ± 2.2**6/633.75 ± 11.6***11.7 ± 4*
MNNG + metal mixture76 ± 0.05***6/623 ± 7.7***13.6 ± 4.6*
Metal mixture + metal mixture31 ± 6.9***6/629.5 ± 5.7***50.5 ± 9.7***

aTreatments were added at concentrations of MNNG 0.5  g/mL, TPA 0.1  g/mL, and metal mixture (As 2  M, Cd 2  M, and Pb 5  M); bpercentage with respect to controls; ctwo experiments with three biological replicates; dtransforming potential (TP) calculated as nmber of transformed foci type III per dish/surviving cells at corresponding sampling day. Statistical analysis, Student’s t-test value *< 0.05, **< 0.01 and ***< 0.001.