Antioxidant Capacity of Flavonoids in Hepatic Microsomes Is not Reflected by Antioxidant Effects In Vivo

Table 4

Effects of 2 weeks repletion with either 100 mg α-tocopherol, quercetin, kaempferol or myricetin/kg diet on indices of muscle damage, lipid peroxidation and antioxidant capacity of vitamin E deficient rats.

Parameter	Dietary inclusion
	−Vitamin E	+dα-tocopherol	+Quercetin	+Kaempferol	+Myricetin
Pyruvate kinase (U/L)	807 ± 76	132±13^∗∗∗	632 ± 98	617 ± 108	742 ± 81
TBARS (nmol/mL plasma)	1.66 ± 0.13	1.13±0.09^∗	2.14 ± 0.51	2.33 ± 0.34	2.08 ± 0.21
TBARS (nmol/mg hepatic protein)	15.3 ± 0.7	2.4±0.6^∗∗∗	13.4 ± 0.5	13.8 ± 0.8	12.5 ± 0.3
Erythrocyte lipid peroxidation (nmol/mgHb)	50 ± 7	3±1^∗∗∗	53 ± 4	63 ± 5	64 ± 4
EPR signal height (RU/mg hepatic protein)	22423 ± 1133	1036±71^∗∗∗	19950 ± 1458	18833 ± 448	17425 ± 1892

Results are mean ± SEM of 6 rats/group. Significantly different from −Vitamin E group (^*𝑃<0.05; ^***𝑃<0.001). TBARS: thiobarbituric acid reactive substances Hb: haemoglobin, EPR: Electron paramagnetic resonance spectroscopy, RU: relative units.