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Oxidative Medicine and Cellular Longevity
Volume 2012 (2012), Article ID 274953, 8 pages
http://dx.doi.org/10.1155/2012/274953
Research Article

Effects of Temperature and Water-Filtered Infrared-A Alone or in Combination on Healthy and Glyoxal-Stressed Fibroblast Cultures

1Institute of Anatomy of the Faculty of Medicine, TU Dresden, 01307 Dresden, Germany
2Medical Sensoring and Monitoring of the Faculty of Medicine Carl Gustav Carus, TU Dresden, 01307 Dresden, Germany
3CRTD/DFG-Center for Regenerative Therapies Dresden-Cluster of Excellence, TU Dresden, 01307 Dresden, Germany

Received 10 August 2012; Revised 25 September 2012; Accepted 25 September 2012

Academic Editor: Elisa Cabiscol

Copyright © 2012 Lilla Knels et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Water-filtered infrared-A (wIRA) radiation has been described as supportive for tissue regeneration. We sought to investigate in detail the wIRA effect at different temperatures in 3T3 fibroblasts that were treated with glyoxal to induce formation of advanced glycation end products (AGEs). Nonirradiated and nonglyoxal-treated cells served as controls. Experiments were carried out over a range of 37–45C with exact temperature monitoring to distinguish between temperature and wIRA effects. Metabolic activity was assessed by resazurin assay. Mitochondrial membrane potential was assessed by JC-1 vital staining. Apoptotic changes were determined by vital staining with annexin V and YO-PRO-1 and determination of subG1 DNA content. Temperature had a dominant effect overriding effects exerted by wIRA or glyoxal treatment. The number of apoptotic cells was significantly higher at 45C, while the percentage of healthy cells was significantly lower at 45C. WIRA irradiation itself or in combination with glyoxal treatment exerted no damaging effects on the fibroblasts at physiological (37–40C) or higher (42–45C) temperatures compared to untreated controls. Temperatures of 45C, which can occur during inappropriate application of infrared irradiation, damage cells even in the absence of wIRA or glyoxal application.