Bicarbonate Plays a Critical Role in the Generation of Cytotoxicity during SIN-1 Decomposition in Culture Medium
Figure 2
HPLC analysis of the time course of SIN-1 decomposition in bicarbonate. HPLC profile of fresh and decomposed SIN-1. SIN-1 (1 mM) dissolved in 10 mM NaHCO3 (pH 9.6) was allowed to decompose for up to 24 h. Aliquots were immediately separated by HPLC on a C18 column. The retention times for SIN-1 and SIN-1C were 3 min and 20 min, respectively. The asterisk indicates the possible peak for SIN-1A, which was detectable immediately before the SIN-1C peak only within the first 1 h. The inset graph shows the relative amounts of SIN-1 and SIN-1C at each time point. Areas corresponding to SIN-1 were normalized to that for , and the areas for SIN-1C were normalized to that obtained for 4-h-decomposed SIN-1. Values are means ± ranges of two independent experiments.
