Bicarbonate Plays a Critical Role in the Generation of Cytotoxicity during SIN-1 Decomposition in Culture Medium
Figure 9
Effect of SIN-1 treatment on GSH content and mitochondrial respiratory enzyme complex activities. Cells in 6-mm dishes were treated with fresh SIN-1 in FCS/RPMI or 2 h SIN-1-decomposed FCS/RPMI (1 mM) for 2 h or 4 h, and the activities of citrate synthase (a; control activity = 114 mU/mg), complex IV (b; control activity = 7.9 k/mg), and complex V (c; control activity = 11.6 mU/mg), and the GSH content (d; control cells, 11.9 nmol/mg) were measured. Values are means ± ranges from two wells in a representative experiment and are expressed as a percentage of the control. n.d.: not detectable.
